Viral Vector Process Development
![Scalable bioreactor system for viral vector process development in cell cultures](/media/MAIN/01-Lab-Academy/Bioprocessing/Header-and-icons/1417396_Header_Bioprocess-Optimization_2220x740_1080.jpg)
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Upstream bioprocess development for viral vector production
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Plasmid production
In the context of viral vector production, plasmids can be adapted to contain the instructions for producing viruses with specific genomes. Through standard plasmid cloning, viruses can therefore be engineered to contain important genes for either gene therapy or vaccination purposes. Viral vectors can be used as a powerful tool for the delivery of this genetic information into mammalian cells. During the plasmid production stage, plasmids are either constructed in the laboratory or selected from existing stock. The type and number of plasmids you require will depend on the viral vector you choose to use and whether you intend to use transient or stable cell lines. Learn more about plasmid production in our webinar!
![845199_730px_x_411px](/media/MAIN/01___lab_acadamy/bioprocessing/photos/845199_730px-x-411px_700.jpg)
Cell expansion and virus production
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Cultivation of adherent cells
Growing adherent cells relies on a growth support matrix. Conventionally, 2D culture systems like flasks and dishes have been used, but as technology has evolved, cultivation in bioreactors provides an alternative option that exploits the benefits they offer in terms of process control and scalability. The use of microcarrier-based culture systems and packed-bed bioreactors filled with Fibra-Cel® Disks are options for cultivating adherent cells in bioreactors.
![Packed-bed bioreactor filled with Fibra-Cel Disks suitable for viral vector production](/media/MAIN/01___lab_acadamy/bioprocessing/photos/1417444_730px-x-411px_700.jpg)
Fibra-Cel® Disks
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Microcarrier-based cell culture systems
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Plasmid transfection, viral vector production, and harvest
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Learn more about transient and stable transfection
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Bioprocessing for AAV vector production
Cells will need to be harvested and the viral vectors isolated for characterization and quantification. An important part of this process is determining the ratio of viral vectors with genomes to those without, known as the full/empty ratio. This can be done using methods including ELISA, electron microscopy, analytical ultracentrifugation (AUC), and high-pressure liquid chromatography (HPLC), each of which has benefits and limitations of its own. For information on AAV vector production workflow in a bioreactor, check out this application note!
![Lab scene showing lab equipment needed for viral vector process development](/media/MAIN/01___lab_acadamy/bioprocessing/photos/1417384_730px-x-411px_700.jpg)
Eppendorf upstream bioprocessing solutions for cell culture
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Small scale bioreactor systems
The DASbox® Mini Bioreactor System and DASGIP® Parallel Bioreactor Systems for cell culture applications are well suited for process development and downscaling approaches.
- Working volume range: 100 mL to 1.5 L
- Parallel processing of up to 24 bioreactors
- Compatible with glass bioreactors and BioBLU® Single-Use Bioreactors
- Suitable for suspension cell culture and cell culture on microcarriers
![Parallel mini bioreactor system which can be used in viral vector process development.](/media/MAIN/01-Lab-Academy/Bioprocessing/Photos/68831_730px-x-411px_700.jpg)
Bench scale bioreactor systems
The bench scale bioreactor systems BioFlo® 120 , BioFlo® 320 , and SciVario® twin facilitate process development and scale-up
- Working volume range: 200 mL to 40 L
- Compatible with glass bioreactors and BioBLU® Single-Use Bioreactors
- Suitable for suspension cell culture and cell culture on microcarriers
- Compatible with packed-bed bioreactors filled with Fibra-Cel® Disks
![Bench-scale bioreactor system which can be used in viral vector process development](/media/MAIN/01___lab_acadamy/bioprocessing/photos/818020_730px-x-411px_700-2.jpg)
Large scale bioreactor systems
For cell cultivation at large scale Eppendorf offers the BioFlo® 720 bioreactor control system.
- Offers scalability from 50 L to 2000 L
- Compatible with the BioBLU® 50c Single-Use Bioreactor
- Compatible with Thermo Scientific® HyPerforma® Single-Use Bioreactors
![Large-scale bioreactor process development which can be used to scale-up viral vector production.](/media/MAIN/01___lab_acadamy/bioprocessing/photos/900420_730px-x-411px_700.jpg)
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